Abstract
Cereblon (CRBN) is a substrate recognition subunit of the CRL4 E3 ubiquitin ligase complex, directly binding to specific substrates for poly-ubiquitination followed by proteasome-dependent degradation of proteins. Cellular CRBN is responsible for energy metabolism, ion-channel activation, and cellular stress response through binding to proteins related to the respective pathways. As CRBN binds to various proteins, the selective pressure at the interacting surface is expected to result in functional divergence. Here, we present two mammalian CRBN datasets of molecular evolutionary analyses. (1) The multiple sequence alignment data shows that positive selection occurred, determined with a dN/dS calculation. (2) Data on co-evolutionary analysis between vertebrate CRBN and related proteins are represented by calculating the correlation coefficient based on the comparison of phylogenetic trees. Co-evolutionary analysis shows the similarity of evolutionary traits of two proteins. Further molecular, functional interpretation of these analyses is explained in ‘Positive selection of Cereblon modified function including its E3 Ubiquitin Ligase activity and binding efficiency with AMPK’ (W. Onodera, T. Asahi, N. Sawamura, Positive selection of cereblon modified function including its E3 ubiquitin ligase activity and binding efficiency with AMPK. Mol Phylogenet Evol. (2019) 135:78-85. [1]).
Highlights
Data accessibilityBiology Molecular evolution Table, Figure Phylogenetic tree acquired using maximum likelihood & neighbor-joining method at MEGA7 software. dN/dS acquired using maximum likelihood & counting method at Selecton server and Datamonkey server
Cereblon (CRBN) is a substrate recognition subunit of the CRL4 E3 ubiquitin ligase complex, directly binding to specific substrates for poly-ubiquitination followed by proteasome-dependent degradation of proteins
(2) Data on co-evolutionary analysis between vertebrate CRBN and related proteins are represented by calculating the correlation coefficient based on the comparison of phylogenetic trees
Summary
Biology Molecular evolution Table, Figure Phylogenetic tree acquired using maximum likelihood & neighbor-joining method at MEGA7 software. dN/dS acquired using maximum likelihood & counting method at Selecton server and Datamonkey server. Biology Molecular evolution Table, Figure Phylogenetic tree acquired using maximum likelihood & neighbor-joining method at MEGA7 software. Analyzed Nucleotide coding sequences were downloaded from NCBI GenBank. Sequences used in this article available at NCBI GenBank (https://www.ncbi.nlm.nih.gov/) via accession number (see Supplementary Table 1). The data contains phylogenetically analyzed CRBN sequences. The sequences were collected from NCBI GenBank (sequence accession numbers available in Supplementary Table 1). Ancestral state of position 366 are illustrated in Fig. 3 estimated using maximum likelihood method. The result for coevolutionary analysis of vertebrate CRBN based on mirror tree method are listed in Table 1 and Fig. 3
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