Abstract
Serotype C and D of Clostridium botulinum produce botulinum toxin complex (TC), which is comprised of botulinum neurotoxin, nontoxic nonhemagglutinin, and hemagglutinins (HAs). The TC is capable of aggregating equine erythrocytes via interaction between one of the HAs, namely HA-33, and sugar chains on the cell surface. This hemagglutination is inhibited by specific sugars. In this data article, we used four TCs from serotype C and D strains. The hemagglutination-inhibiting effects of 18 sugars and 8 glycoproteins were studied. The purified TC was mixed with the sugar to enable binding of the sugar to the TC; then, the erythrocytes were added to the mixture. Specific binding between the sugar and TC resulted in inhibition of cell aggregation. Here, data illustrating the inhibitory effects of various sugars and glycoproteins against hemagglutination induced by TC of C. botulinum serotypes C and D are presented.
Highlights
Data describing inhibitory profiles of sugars against hemagglutination by the botulinum toxin complex of Clostridium botulinum serotypes C and D
Table Protein purification, SDS-PAGE, hemagglutination assay, and hemagglutination inhibition assay Raw Toxin complexes produced by four strains of C. botulinum were purified from the culture media of each strain
The data presented illustrate the specific inhibitory effect exerted by 18 sugars and 8 glycoproteins on hemagglutination by the toxin complex (TC) of serotypes C and D of C. botulinum
Summary
L-TC was purified from the culture of C. botulinum C-St [1], C-Yoichi [2], D-CB16 [3], and D-1873 [4] as described in the cited studies. The residues essential for the sugar-recognition in the BoNT molecule are covered with other components of the L-TC [6,7]; the cell binding of the L-TC dominantly depend on the HA-33 components that exposed to the most outside of the molecule [7]. Previous studies have shown that C-St and D-CB16 L-TCs exert the hemagglutination activity against equine erythrocytes, whereas C-Yoichi and D-1873 L-TCs exhibited no, or very low, hemagglutination titer against equine erythrocytes. C-Yoichi and D-1873 L-TCs were found to exhibit hemagglutination titer against equine erythrocytes treated with neuraminidase [2,4]. Normal erythrocytes were used to study hemagglutination by C-St and D-CB16, and neuraminidase-treated erythrocytes were used to study hemagglutination by the C-Yoichi and D-1873 strains
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