Abstract
We present Dynamic Adaptive Scattering Compensation Holography (DASH) [1], a novel algorithmic approach for indirect wavefront sensing in nonlinear scanning microscopy that is suited for imaging into highly scattering media. DASH utilizes a single phase-only SLM for sculpting a continuously updated aberration corrected beam and a test beam with a particular wavefront shape. The test beam is phase-stepped to evaluate its importance and optimal phase and then added to the corrected. We demonstrate DASH for 2-photon fluorescence imaging of GFP expressing microglia cells in fixed and living mouse hippocampal brain tissue at over 500 µm depth. 1. May, Molly A., Nicolas Barré, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte, and Alexander Jesacher. "Fast holographic scattering compensation for deep tissue biological imaging." Nature Communications 12, no. 1 (2021): 1-8.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
