Abstract
Two plants, Ornithogalum caudatum and Scilla siberica, with contrasting types of heterochromatin, were employed to investigate the effect of prestaining with actinomycin D on the differential DAPI fluorescence in nuclei and chromosomes. Actinomycin D-pretreated preparations exhibited in both plants a significantly lower overall fluorescence intensity than DAPI controls. In O. caudatum the fluorescence properties of DAPI-bright heterochromatin remained qualitatively unchanged, but in S. siberica chromosomes the differential fluorescence of DAPI-negative heterochromatin disappeared or occasionally was reversed. It is shown that the observations cannot be interpreted simply as a result of base specific drug-DNA interaction.
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