植物生長調節劑對大水蚤（Daphnia magna）之毒性影響與胚胎毒性試驗探討

Abstract

Plant growth regulators (PGRs) is one kind of the pesticides, and they have been widely used as crop, flowers and turf. The main function is regulate the growth of the plant. Only few plant growth regulators be studied research in the environmental and biological research at present, others have no animal's toxicity test yet. The present study were performed acute toxicity test, embryo development test, and recorded the rate of target organ been effected by the chemicals to evaluate the toxicities of atonik, cytokinin, gibberellic acid, ethephon and paclobutrazol by neonate and embryo of Daphnia magna, respectively. In acute toxicity test, the 48 hour LC50 value of atonik, cytokinin, gibberellic acid, ethephon and paclobutrazol were 18.88 mg/L, 1.51 mg/L, 14.66 mg/L, 125.32 mg/L and 25.64 mg/L, respectively. In embryo development test, the 72 hour EC50 value of atonik, cytokinin, gibberellic acid, ethephon and paclobutrazol were 23.98 mg/L, 0.58 mg/L, 16.71 mg/L, 102.62 mg/L and 0.07 mg/L, respectively. The results indicate that the cytokinin is toxic to neonate, the paclobutrazol is toxic to embryo, and the ethephon is mild toxic to neonate and embryo of Daphnia magna. It was also observed that the malpighian tube of Daphnia magna is the more sensitive than the other organs. The present study indicate that the embryo development test with target organ effected rate were more sensitive than traditional acute toxicity test. Suggest that the method may be apply in environmental toxicants evaluation.