DanHong Injection inhibits the development of primary abdominal aortic aneurysms in apoE knockout mice

Abstract

Clinical observations indicate that DanHong Injection (DHI) can increase blood flow and reduce various syndromes in patients with cardiovascular disease. However, it still needs to define the function of DHI and the involved mechanisms in details, such as the protective effect on the development of primary abdominal aortic aneurysms (AAAs). In this study, we determined whether DHI is able to inhibit AAA in apoE knockout (apoE−/−) mice. Thirty apoE−/− male mice on high-fat diet (0.5 % cholesterol, 21 % fat) were randomly divided into two groups and received i.p. injection of saline (100 μL/day) and DHI (100 μL/day), respectively, for 16 weeks. At the end of experiment, we determined the development of atherosclerosis in en face aorta and aneurysms, pathological morphology of arterial wall, and serum lipid levels. We also determined the expression of monocyte chemoattractant protein-1 (MCP-1), MMP-2, and MMP-9 mRNA in aortic wall using real-time RT-PCR. Our results indicated that high-fat diet induced the development of AAAs in apoE−/− mice, but the induction was totally blocked by DHI (P < 0.01). The result of staining of abdominal aortic cross sections showed that DHI maintained the collagen content in arterial wall, thereby preventing the animals from the development of AAA. Although DHI had little effect on serum total- and LDL-cholesterol levels, it reduced the expression of MCP-1, MMP-2, and MMP-9 mRNA in aortic wall (P < 0.01). Taken together, our study suggests that DHI can inhibit the high-fat diet-induced AAA formation. The inhibitory effects may be related to the maintenance of the collagen content and inhibition of expression of AAA-related genes. Our study may suggest a new application of DHI in clinics.