Abstract
Ultraviolet (UV) irradiation causes damage in skin by generating excessive reactive oxygen species (ROS) and induction of matrix metalloproteinases (MMPs), leading to skin photoageing. Dandelion extracts have long been used for traditional Chinese medicine and native American medicine to treat cancers, hepatitis, and digestive diseases; however, less is known on the effects of dandelion extracts in skin photoageing. Here we found that dandelion leaf and flower extracts significantly protect UVB irradiation-inhibited cell viability when added before UVB irradiation or promptly after irradiation. Dandelion leaf and flower extracts inhibited UVB irradiation-stimulated MMP activity and ROS generation. Dandelion root extracts showed less action on protecting HDFs from UVB irradiation-induced MMP activity, ROS generation, and cell death. Furthermore, dandelion leaf and flower but not root extracts stimulated glutathione generation and glutathione reductase mRNA expression in the presence or absence of UVB irradiation. We also found that dandelion leaf and flower extracts help absorb UVB irradiation. In addition, dandelion extracts significantly protected HDFs from H2O2-induced cellular senescence. In conclusion, dandelion extracts especially leaf and flower extracts are potent protective agents against UVB damage and H2O2-induced cellular senescence in HDFs by suppressing ROS generation and MMP activities and helping UVB absorption.
Highlights
IntroductionSkin is more transparent, loose, and fragile. One of the most damaging actions on skin is from solar radiation, especially from its ultraviolet (UV) component, leading to both clinical and histologic damage on human skin [1, 2]
When skin is aged, skin is more transparent, loose, and fragile
We found that dandelion leaf and flower extracts but not root extracts are potent protective agent against UVB damage and H2O2induced cellular senescence in human dermal fibroblasts (HDFs) by suppressing reactive oxygen species (ROS) generation and matrix metalloproteinases (MMPs) activities
Summary
Skin is more transparent, loose, and fragile. One of the most damaging actions on skin is from solar radiation, especially from its ultraviolet (UV) component, leading to both clinical and histologic damage on human skin [1, 2]. MMPs-mediated degradation of the collagenous extracellular matrix (ECM) accounts for most of the connective tissue damage that occurs in photodamaged skin [3, 4]. UV irradiation consists of three components, UVA, UVB, and UVC. Whereas UVA and UVB reach the earth in sufficient amounts to damage the skin, UVC is almost completely absorbed by the ozone layer [2, 6]. UVB is damaging, as it penetrates the epidermis and the upper part of the dermis, where it damages fibroblast cells and leads to sunburn, photoageing, and skin cancer [7, 8]. People often use sunscreen to protect the skin from UV damaging from the sun, and sunscreen absorbs UV rays and prevents them from penetrating the skin. It is necessary to find effective, safer, and environmentally friendly nature products for antiageing and UV protection
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
