Damage to synaptonemal complex structure and peculiarities of selection of mouse spermatocytes I at response to drug administration

Abstract

Using immunocytochemistry methods, the structure of synaptonemal complexes (SC) of chromosomes in spread nuclei of primary spermatocytes of mice at 1, 10, and 36 days after the 10-day intraperitoneal administration of antibacterial preparations of three pharmacological groups: furacilin, an antiseptic derivative of nitrofuran; cifran, an antibiotic from the group of fluoroquinolones; and sextaphage, a polyvalent piobacteriophage was investigated. The maximal number of disturbances in the structure and behavior of synaptonemal complex was revealed on the first day after the end of preparation administration. On days 10 and 36, the total number of disturbances in SC structure decreased gradually. On the first day after the end of the administration of cifran and sextaphage in 41.8 and 25% of nuclei, respectively, the fragmentation of synaptonemal complexes was revealed and, in males to whom furacilin had been administered, the fragmentation of synaptonemal complexes was identified in 100% of nuclei. Multiple chromosome fragmentation is a meiotic disaster and results in the degeneration of cells without enabling the mechanism ofpachytene arrest. The features of pachytene arrest were revealed in the nuclei of primary spermatocytes with the disturbances of chromosomes pairing. After the administration of sextaphage, circle structures released from the lateral elements of SC and are dyed with antibodies to SCP3 protein.