Abstract
Fixed specimens of human blood cells have been bombarded by 200 keV triton beams with fluences up to 7 × 10 16 tritons/cm 2. The target chamber was filled with 0.5 atm of helium for heat transfer from the target. High magnification phase contrast photo-micrographs and low resolution scanning electron micrographs of the cells are used to show morphological details of radiation damage. Differences in images of the same radiation-damaged cells made with these two microscopy techniques are described. A fluence of 4 × 10 16 tritons/cm 2 was found to strip cytoplasm from white blood cells leaving their nuclei naked but intact. The same fluence caused relatively minor damage to red blood cells. Limits imposed by radiation damage on biomedical information that may be obtainable using triton bombardment to localize deuterated tracer metabolites in individual cells are discussed. A brief comparison with radiation damage observations in electron microscopy is also given.
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