Abstract
Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms. If the interaction between many living microbes can be controlled and accelerated via remote assembly, a cultivation process requiring a few days can be ommitted, thus leading to a reduction in the time needed to analyze the bacterial functions. Here, we show the rapid, damage-free, and extremely dense light-induced assembly of microbes over a submillimeter area with the “bubble-mimetic substrate (BMS)”. In particular, we successfully assembled 104–105 cells of lactic acid bacteria (Lactobacillus casei), achieving a survival rate higher than 95% within a few minutes without cultivation process. This type of light-induced assembly on substrates like BMS, with the maintenance of the inherent functions of various biological samples, can pave the way for the development of innovative methods for rapid and highly efficient analysis of functions in a variety of microbes.
Highlights
Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms
Bacterial symbiotic mechanism is a crucial issue for human health and environmental problems; for instance, quorum sensing[1,2,3,4,5,6,7] and probiotics of microbes[8,9,10,11,12] have attracted significant attention
In this study, we succeeded in achieving large-scale and damagefree light-induced assembly of a vast number of microbial cells at the solid–liquid interface on a substrate without bubble generation under laser irradiation using a designed bubble-mimetic substrate consisting of a submillimeter polymer particle as an imitation bubble
Summary
Rapid evaluation of functions in densely assembled bacteria is a crucial issue in the efficient study of symbiotic mechanisms. In order to develop a method for achieving highly dense, large-scale, and damage-free light-induced assembly of dispersoids and to clarify the underlying physicochemical mechanism of the assembly, we attempted to produce a bubble-mimetic substrate consisting of a polystyrene particle (an imitation bubble as large as a submillimeter light-induced bubble) fixed on a glass substrate Using this substrate, we performed experiments to remotely control the assembly of lactic acid bacteria, Lactobacillus casei (L. casei), as an example of intestinal bacteria, as biological dispersoids by means of laser irradiation
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