Daily variations in monoamine turnover in the brain of the ring dove ( Streptopelia risoria)

Abstract

A method for measuring the concentration and turnover of serotonin, dopamine and noradrenaline in small amounts of brain tissue was validated for the ring dove ( Streptopelia risoria). Turnover rates of the catecholamines calculated as the rate of depletion after tyrosine hydroxylase inhibition agreed well with turnover rate measured as rate of accumulation after inhibition of monoamine oxidase at 2.5 h, but not 5 h, after drug administration. Using the monoamine oxidase inhibitor, pargyline, turnover of serotonin, dopamine and noradrenaline were estimated in the same tissue. Both the concentration and turnover of the amines in hypothalamus, paleostriatum and hyperstriatum fluctuated over a 24 h period. Serotonin turnover was greatest during the middle of the dark period in all three tissues. In contrast, for both dopamine and noradrenaline the highest turnover was observed during the light period and the lowest during the dark period.