Daily activity measures and milk yield immediately before and after a fertile estrus and during the period of expected return to estrus after insemination in dairy cows

Abstract

The objective of this study was to characterize changes in milk yield and other physical measures during a 7-d periestrual period encompassing estrus (d 0) and during a 16-d period of expected return to estrus beginning at d 17 after artificial insemination (AI) until pregnancy status was determined on d 32. Lactating dairy cows milked thrice daily were fitted with CowManager SensOor ear tags (Agis) capable of assessing real-time eating, rumination, resting, high activity (estrus), ear-surface temperature, and heat alerts. Data were uploaded to the cloud, downloaded daily into Excel (Microsoft Corp.) spreadsheets, averaged to produce daily means for each activity, and analyzed as repeated measures relative to estrus or to d 17 after AI. Daily milk was unchanged during the periestrual period but was greater in nonpregnant cows that failed to return to estrus (NP-NR) during d 21 through 26 compared with NP cows that returned to estrus (NP-R) and pregnant (PREG) cows during that same period. Daily ear-surface temperature was greater during d 1 to 3 compared with d 0 and averaged 0.6 to 1.7°C greater from d 17 through 32 in NP-NR cows compared with NP-R and PREG cows. Daily rumination and resting times reached nadirs on d 0, with decreases occurring 48 h before estrus. Both rumination and resting times increased by 25 or 81% on the day after estrus, respectively. Rumination and resting times were less in NP-R cows during d 22 through 26 compared with NP-NR and PREG cows. In contrast, daily eating time was greatest on the day of estrus compared with 3 d before and after estrus. The NP-R cows spent more time eating during d 17 through 32 compared with NP-NR and PREG cows. High activity increased by 97% during 48 h before estrus, peaked at estrus, and decreased to a constant level during d 1 through 3. The NP-R cows had greater high activity on d 22 through 26 compared with NP-NR and PREG cows. We conclude that resting and rumination activity decreased to daily nadirs, whereas eating and high activity peaked on the day of estrus. Fertile estrus was associated with 12% greater high activity, 11% less resting time, and 6% less rumination time. In addition, cows that returned to estrus after AI had greater daily eating and high activity times and less rumination and resting time during the period of expected return to estrus after AI compared with pregnant cows and cows failing to return to estrus.