Abstract
High-purity diacylglycerol (DAG) oil was enzymatically obtained from palm oil deodoriser distillate (PODD). Free fatty acids from PODD were esterified with glycerol (2.5:1 fatty acid to glycerol molar ratio) in the presence of 10% (w/w of oil) Rhizomucor mieheilipase (Lipozyme RM IM) and 30% (w/w of oil) molecular sieves and incubated for 6h at 65 °C in a 50 mL bioreactor. After esterification, the products were deacidified by alkaline extraction and the DAG oil was further purified by silica column chromatography. After purification, up to 85.2% (w/w) of DAG was obtained. DAG profile, fatty acid composition, iodine value, slip melting point and thermal profiles were determined.
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