D-Map: Random Walking on Gene Network Inference Maps Towards differential Avenue Discovery.

Abstract

Differential rewiring of cellular interaction networks between disease and healthy state is of great importance. Through a systems level approach, malfunctioned mechanisms that are absent in the normal cases, may enlighten the key-players in terms of genes and their interaction chains related to disease. We have developed D-Map, a publicly available user-friendly web application, capable of generating and manipulating advanced differential networks by combining state-of-the-art inference reconstruction methods with random walk simulations. The inputs are expression profiles obtained from the Gene Expression Omnibus and a gene list under investigation. Differential networks may be visualized and interpreted through the use of D-Map interface, where display of the disease, the normal and the common state can be performed, interactively. A case study scenario concerning Alzheimer's disease, as well as breast, lung, and bladder cancer was conducted in order to demonstrate the usefulness of the proposed methodology to different disease types. Findings were consistent with the current bibliography, and the provided interaction lists may be further explored towards novel biological insights of the investigated diseases. The DMap web-application is available at: http://bioserver-3.bioacademy.gr/Bioserver/DMap/index.php.