D - lactic acid production from waste paper by recombinant Escherichia coli JH13

Abstract

Waste paper as a new kind of biomass resource was selected as the research object to ferment and produce D-lactic acid in this paper. The components of raw materials were compared between waste office paper and waste newspaper, which were pretreated by shredding, 3% H2SO4 and enzymatic hydrolysis. The hydrolysate could be used as single carbon source for lipid fermentation by Escherichia coli JH13. Shake-flask fermentation was carried out to produce D-lactic acid by Escherichia coli JH13 using the hydrolysate of waste office paper and waste newspaper as carbon source. Fermentation with waste office paper and waste newspaper hydrolysate as carbon source achieved a D-lactic acid production of 35.86 ± 0.62 g/L, which was 39.8% higher than the D-lactic acid production (25.64 ± 0.48 g/L) with waste newspaper hydrolysate as carbon source.