D-Galaktoz ile İndüklenen Farelerde Yaşlanma Modelinin Nefropatisi Üzerine Mirisitrin ve Vitamin E’nin Koruyucu Etkileri

Abstract

Aim: Aging occurs in cells and tissues due to oxidative stress in physiological conditions. D-galactose (DG) is widely used to cause aging in animal studies. In this study, the renal protective effects of myricitrin and vitamin E in the aging mice model induced by DG was evaluated. Material and Methods: Subcutaneous DG injection was used for induction of the aging model. 72 female mice were randomly divided into six groups: All groups were received DG at 500 mg/kg/d for six weeks. In the last 28 days, the groups treated with myricitrin subcutaneously received 5, 10, and 20 mg/kg/d, and the vitamin E group received 100 mg/kg/d by gavage. Urine and plasma albumin, BUN, creatinine levels, MDA, TAC, and kidney histological changes were evaluated. Results: Plasma albumin was significantly decreased (p=0.001), but a significant increase in urine albumin (p=0.001), BUN (p<0.001), and creatinine (p=0.010) levels was observed in the DG group when compared with the control. Also, a significant increase in MDA levels (p=0.002) along with a significant decrease in TAC (p=0.012) was observed. Histopathological changes such as congestion of erythrocytes (p<0.001), infiltration of inflammatory cells (p<0.001), and proximal tubule cell damage (p=0.004) significantly increased, while glomerulus diameter significantly decreased (p=0.038) in comparison to the control. Administration of myricitrin and vitamin E showed a significant ameliorative effect on all studied variables. Conclusion: The improvement effects of myricitrin on DG-induced kidney damage was approximately equivalent to vitamin E. Myricitrin and vitamin E could have beneficial effects on the nephropathy of aging model.