Abstract
目的观察CD138-CAR-T细胞对人多发性骨髓瘤(MM)细胞株RPMI8226和U266细胞的杀伤作用,探讨泊马度胺对CD138-CAR-T细胞及其杀伤作用的影响。方法采用CFSE/7-AAD双标法检测CD138-CAR-T细胞及其联合泊马度胺对RPMI8226、U266细胞的杀伤活性。ELISA法检测CD138-CAR-T细胞分泌IFN-γ的变化。结果CD138-CAR-T细胞作用18 h后,对RPMI8226、U266细胞的杀伤活性分别为(55.2±3.9)%、(85.1±2.4)%,对照组分别为(7.0±1.5)%、(12.5±2.1)%,差异均有统计学意义(P值均<0.01);与CD138-CAR-T细胞组比较,CD138-CAR-T细胞联合泊马度胺(2.5 µg/ml)作用18 h后,对RPMI8226、U266细胞的杀伤活性差异无统计学意义(P值均>0.05)。与CD138-CAR-T细胞组比较,CD138-CAR-T细胞和MM细胞共培养组IFN-γ分泌水平显著增高;与共培养组比较,加入泊马度胺后能显著促进IFN-γ的释放,差异均有统计学意义(P值均<0.01)。结论CD138-CAR-T细胞对MM细胞及耐药细胞株均有明显的杀伤作用;其与MM细胞共培养能促进后者IFN-γ的分泌;泊马度胺能促进CD138-CAR-T细胞分泌IFN-γ。
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