Abstract
Introduction Pulpotomy is a therapeutic procedure that consists of the surgical amputation of coronally inflamed pulp. The wounded surface of the radicular pulp is treated with a medicament or dressing agent to promote healing or to cause fixation of the underlying tissue. This in-vitro study assessed the cytotoxicity of different formulations of omega 3 as pulpotomy agents compared with formocresol (FC) on peripheral blood mononuclear cells. Materials and methods Different formulations were tested using 96-well plates. The optical density of neutral red extract was measured using spectrophotometer, using blanks which contain no cells as a reference. The data were analyzed by Kruskal–Wallis test at the level of significance set at 0.001. Results The results showed that FC, ZnO, and ZnO/Tw/Eth had cytotoxic effects with lower survival rate of cells. There was absence of cytotoxicity after incubation with ZnO/Tw/Eth/ω3, Tw/Eth, MC, and MC/Tw/Eth in comparison with control. Moreover, there was no cytotoxic effect in cases of MC/Tw/Eth/ω3 and Tw/Eth/ω3, which showed higher survival rate of normal cells. Conclusion Omega 3 has no cytotoxic effects on viable cells, and it diminishes the toxic effect of ZnO when formulated with it and enhanced the effect of methylcellulose when compared with the cytotoxic FC.
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