Cytotoxicity of Uncoated Ferroferric Oxide Nanoparticles in Vascular Endothelial Cells

Abstract

Ferro ferric oxide(Fe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> ) nanoparticles have found application as contrast agents for magnetic resonance imaging and as targeted delivery of drugs. Their stabilization as carrier systems remains a problem. The cytotoxicity of vascular endothelial cells induced by uncoated Ferro ferric oxide nanoparticles were investigated in this study. EA.hy926 cells were incubated with fresh medium containing uncoated Fe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> nanopartilces in different concentrations(0μg/ml, 2μg/ml, 20μg/ml, and 100μg/ml), after 12 hours cell viability was examined with CCK-8 assay and LDH release assay. The preliminary toxic mechanism of cytotoxicity was determined via MDA levels. The Ferro ferric Oxide nanopartilces can cause apparent inhibition effect on the growth of the EA.hy926 cells at the concentration 100μg/mL, as well as MDA levels are significant in this group. Oxidative damage may be one of its toxic mechanisms. It would provide an experimental foundation for security application of Ferro ferric oxide nanoparticles.