Abstract
The cytotoxicity of fresh samples of AH26 and AH Plus were tested using the 51 Crrelease method at 12 and 72 h. Heteroploid L929 mouse fibroblasts were used as target cells. For the 51 Cr-release assay, cells were labeled with 51 Cr before exposure to the test materials, and the radioactivity in the supernatant was counted with a gamma particle counter. The difference in cytotoxicity between the two materials was evaluated using the Wilcoxon Sum-Rank Test. The difference between AH26 and AH Plus was not statistically significant at 12h (p=0.8345) and at 72h (p=0.676). Both AH26 and AH Plus had gamma particle readings that were significantly lower than the positive control (formocresol) and significantly higher than the negative control (labeled, but material free cell suspension), as assessed by Wilcoxon Sum-Rank Tests.
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