Abstract
Herbal medicine has been in use for centuries for a wide variety of ailments; however, the efficacy of its therapeutic agents in modern medicine is currently being studied. Curcuminoids are an example of natural agents, widely used due to their potential contribution in the prevention and treatment of cancer. In this study, the three main compounds of curcuminoids—curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin—were determined by reversed-phase high performance liquid chromatography (HPLC) to quantify total content in a mixture. Subsequently, the effect of the three curcuminoids, employed as one sample, was evaluated, to study the proliferation, apoptosis, cell cycle, and migration of the human ovarian cancer cell line SKOV-3. The results reveal that curcuminoids inhibit the proliferation of SKOV-3 cells with concentration- and time-dependent mechanisms. The morphological analysis of the treated SKOV-3 cells showed a typical apoptotic phenotype—cell shrinkage and membrane blebbing in a dose-dependent manner. In addition, flow cytometry demonstrated an increase in apoptosis with an IC50 of 30 µM curcuminoids. The migration of SKOV-3 cells was also inhibited, reflected by a decrease in wound area. Furthermore, the curcuminoids were found to have no stimulation effect on the expression of cytokines TNF-α and IL-10. These results suggest that a curcuminoid mixture can effectively suppress epithelial cancer cell growth in vitro by inducing cellular changes and apoptosis.
Highlights
Natural remedies have been used since ancient times, and are still in use today
The results indicate that exposure of SKOV-3 cells to curcuminoids for various periods markedly changed the cell cycle phase, compared to the untreated cells
We found that curcuminoids have potent anticancer effects against SKOV-3 cells, one of the most drug-resistant ovarian cancer cell lines
Summary
Natural remedies have been used since ancient times, and are still in use today. In addition to being a vital part of traditional herbal medicine—due to their effectiveness and minimal side-effects—they areSci. Natural remedies have been used since ancient times, and are still in use today. Turmeric has been receiving increased attention from researchers attention from researchers due to its anticancer, antibacterial, antiseptic, antioxidatory, and antidue to its anticancer, antibacterial, antiseptic, antioxidatory, and anti-inflammatory activity, as well as inflammatory activity, as well as its role in treating autoimmune diseases. Turmeric is an orangeits role in treating autoimmune diseases. Turmeric is an orange-yellow pigment extracted from the yellow pigment extracted from the rhizomes of Curcuma longa, a green leafy plant from the rhizomes of Curcuma longa, a green leafy plant from the Zingiberaceae family.
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