Cytotoxicity of silver nanoparticles green-synthesized using Olea europaea fruit extract on MCF7 and T47D cancer cell lines

Abstract

To develop a more environmentally friendly, low-cost, energy-efficient, and non-toxic strategy for producing silver nanoparticles (AgNPs) for cancer therapy, in this study, we established a green synthesis method using Olea europaea fruit extract (OFE) as a reducing and stabilizing agent for the AgNPs. The AgNPs were then characterized using relevant spectroscopy and other analyses, and their cytotoxicity on two breast cancer cells, MCF7 and T47D, were evaluated. The AgNPs exhibited considerable anticancer potential against both MCF7 and T47D cells, inhibiting their proliferation more efficiently than the OFE. The AgNPs-OFE was highly cytotoxic against the T47D cancer cell line with a 50 % inhibitory concentration (IC50) of 77 µg/mL. This study demonstrates the feasibility of using OFE in green synthesizing stable AgNPs with an acceptable particle size and shape and other desirable characteristics, including anticancer activities against the selected in vitro breast cancer cell model. The findings demonstrate the encouraging biomedical and therapeutic application of the Plant-derived AgNPs, which has clinical and academic relevance and warrants further investigation.