Abstract
Today it is generally accepted that most bonding agents are cytotoxic. In this study the relative cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells were investigated. Materials and Methods. Near-confluent mouse 3T3 fibroblast cells were exposed to Dulbecco Modified Eagle’s Medium containing extractions from the seven different bonding agents. The cell survival rate was then determined using the standard MTT assay. Results. The cell survival rate ranking is: iBond (94%) < Gbond (78%) < Xeno V (71%) < Adper Easy Bond (63%) < Xeno V+ (61%) < Adper Scotchbond SE (33%) < XP Bond (32%). Part A of Adper Scotchbond SE had a survival rate of 35% and part B 38%. These two parts did not differ significantly. Adper Scotchbond SE and XP Bond do not differ significantly. While Xeno V+, Xeno V and Adper Easy Bond do not differ. (p < 5%; Tukey-Kramer Multiple-Comparison Test). Conclusion. All of the tested adhesive bonding agents were cytotoxic with survival rate of 3T3 cells between 94% to 31%. Of the 7 bonding agents tested iBond was found to be only slightly toxic and by far the least toxic. The two bonding agents (XP Bond and Adper Scotchbond SE) containing UDMA plus TEGDMA plus HEMA plus camphorquinone were found to be the most toxic.
Highlights
Knowledge of the biocompatibility of dental materials is important so as to prevent hazardous effects on the surrounding tissues where it is placed
All of the tested adhesive bonding agents were cytotoxic with survival rate of 3T3 cells between 94% to 31%
The Box and Whisker plots (Figure 2) were used to compare distributions resulting from the different survival rates of the 7 bonding agents
Summary
Knowledge of the biocompatibility of dental materials is important so as to prevent hazardous effects on the surrounding tissues where it is placed. In an attempt to simplify the bonding technique, manufacturers over the years strived to decrease the number of steps needed to accomplish successful bonding. Twostep-etch and rinse and one step self-etch bonding agents were introduced. They gained rapid popularity because of their relative ease of use [4]. Available dental bonding agents which are used to bond resins to enamel and dentin differ in composition as well as proportions there-off. Research done on the biocompatibility of dental bonding agents [5] and the ingredients showed that many of the constituents to be cytotoxic [6]. The cytotoxic effects of monomers have been reported in several in vitro studies [2,7,8,9,10,11,12,13,14,15,16]
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