Cytotoxicity of root canal sealers on endothelial cell cultures

Vagner José Medeiros Martins,Rivail Antônio Sérgio Fidel,Teresa Cristina Ávila Berlinck,Renata Ximenes Lins

doi:10.1590/0103-6440201302090

Vagner José Medeiros Martins, Rivail Antônio Sérgio Fidel + Show 2 more

Open Access

https://doi.org/10.1590/0103-6440201302090

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Abstract

This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

Highlights

This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line
Cell culture refers to culture derived from scattered cells removed from the original tissue, a primary culture, or a cell line that had already been established in culture by enzymatic, mechanical or chemical disruption [9]
At the 12-h analysis the GuttaFlow had the least cytotoxicity with a mean absorbance of 0.055, followed by Sealer 26 (0.038)

Summary

Introduction

This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO2 and 100% humidity. Among the various recommended methodologies for assessing biocompatibility and cytotoxicity of dental materials at different levels of research, in vitro cell culture tests are part of the initial protocols most widely used by some researchers [3,4,5,6,7]. ECV304 endothelial cells are obtained from the human umbilical cord vein This line is characterized by a monolayered growth pattern, high proliferative potential, with no growth factor-specific requirement [11]

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