Abstract
Bioactive natural compounds derived from plants are the source for the development of new drugs. Numerous in vitro studies have explored the anti-inflammatory effect of eurycomanone and fargesin, derived from Eurycoma longifolia and Flosmagnoliae, respectively. However, before anti-inflammatory investigation is conducted, it is important to obtain the safe doses of these compounds to ensure the validity of the anti-inflammatory results. Therefore, the present study was aimed to investigate the cytotoxicity of eurycomanone and fargesin towards macrophage RAW 264.7. cells to determine the safe doses of these compounds. Different concentrations of eurycomanone and fargesin were subjected to RAW 264.7 cells. The cytotoxicity of the compounds was evaluated by MTT assay and 50% inhibitory concentration (IC50) of these compounds was determined. Morphological changes of RAW 264.7 cells upon exposure to these compounds were also observed. Eurycomanone exhibited its cytotoxic effect by reducing RAW 264.7 cell viability dose-dependently with the IC50 of 94.17 µM. Meanwhile, fargesin had slight cytotoxicity towards RAW 264.7 cells with the IC50 of 173.5µM. Eurycomanone was more cytotoxic towards RAW 264.7 cells compared to fargesin. In conclusion, eurycomanone and fargesin at concentration up to 25 µM, was not toxic to the RAW 264.7 murine macrophages cells and the findings can be applied in the future anti-inflammatory study.
Highlights
Nature isthe major source of bioactive compounds that are used in both traditional and modern medicines
Eurycomanone was more cytotoxic towards RAW 264.7 cells compared to fargesin
Eurycomanone, quassinoids from E. longifolia, with concentration ranging from 0 μM to 200 μM concentration was subjected to macrophage RAW 264.7 cells and the cell viability was assessed using MTT assay
Summary
Nature isthe major source of bioactive compounds that are used in both traditional and modern medicines. Many drugs have been developed directly from bioactive natural compounds and plants are the most explored resourcesfor the development of new drugs and pharmacological studies [1,2,3,4]. Drugs originated from plants used in treatment as anti-inflammatory, anticancerous, and analgesic, include acetylsalicylic acid (Aspirin), digoxin, paclitaxel, vincristine and morphine.For instance, Aspirin, is naturally occurring polyphenol that can be found in Salix sp. Most of the studies on E. longifolia focus on the anticancerous, antiinflammatory and male fertility enhancement effect of the active compounds called eurycomanone[7,8,9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
