Year-end Sale % OFF 
Abstract
This study investigated the cytotoxicity of combined mycotoxins of ochratoxin A (OTA), zearalenone (ZEA), and/or α-zearalenol (α-ZOL). The cytotoxicity of two mycotoxin combinations (two two-toxin combinations and one three-toxin combination) on human Hep G2 cells was evaluated using a tetrazolium salt (MTT) assay and isobologram analysis. Our results demonstrated significant cytotoxic effects of the two-toxin combination and the three-toxin combination on Hep G2 cells in a time- and concentration-dependent manner. The combination indexes (CI) were 2.73–7.67 for the OTA+ZEA combination and 1.23–17.82 for the OTA+α-ZOL combination after 24h, 48h, and 72h of exposure at all inhibit concentration (IC) levels (IC10–IC90), indicating an antagonism. The CIs of the ZEA+α-ZOL combination were 1.29–2.55 after 24h and 72h of exposure (IC10–IC90), indicating an antagonism. The CIs of the ZEA+α-ZOL combination were 0.74–1.68 after 48h of exposure, indicating synergism (IC80–IC90), additive effects (IC50–IC70), or antagonism (IC10–IC40). For the OTA+ZEA+α-ZOL combination, the CIs were 1.41–14.65 after 24h, 48h, and 72h of exposure (IC10–IC90), indicating an antagonism.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
