Cytotoxicity/genotoxicity: The application of cell culture techniques to the measurement of marine sediment pollution

Abstract

Fish cell cultures were used to determine whether in vitro aquatic animal cell culture systems were capable of detecting pollution in the marine environment. Cells derived from rainbow trout gonad (RTG-2) and bluegill fry tissues (BF-2) were used as model cell systems to measure cytotoxicity and genotoxicity following exposure to Puget Sound sediment extracts, benzo( a)pyrene and MNNG. Sediment was collected from several sites within Puget Sound known to be contaminated with compounds such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, chlorinated hydrocarbons and heavy metals. Each of the sediment samples was extracted with organic solvents and added to cultures of the two model cell systems in DMSO. Following exposure the cultures were evaluated for cell death, mitotic inhibition, stimulatory effects, and chromosomal damage. These cell cultures responded to the sediment extracts much as they did to known mutagenic/carcinogenic chemicals which were used as model compounds. Those sediments known to be contaminated, based on chemical analysis and historical use patterns were also found to be qualitatively and quantitatively more toxic than were sediments from areas which were relatively unaffected by human activity. The results show that in vitro cell systems are capable of detecting pollution in the marine environment and have the potential of being powerful tools in aquatic toxicology in conjunction with whole animals or as a method of screening materials prior to in vivo testing.