Abstract
ObjectiveTo evaluate the cytotoxicity, genotoxicity and antibacterial activity of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol (FAR). DesignThe cytotoxicity (% of cell viability) was evaluated by MTT assay and the genotoxicity (% of DNA in the tail) was evaluated by Comet assay. Root canal disinfection with different irrigating protocols was evaluated ex vivo in human teeth contaminated with Enterococcus faecalis for 21days. Three microbiological samples were collected: initial (after contamination); post-irrigation (after irrigation); and final (after 7days). After each sample, the number of log 10 CFU mL−1 was determined. Statistical analyses was performed using two-way ANOVA and Bonferroni post-hoc tests for MTT assay, Kruskal-Wallis and Dunn post-hoc tests for Cometa and antibacterial assays (α=0.05). ResultsThe MTT assay showed that AgNPs and FAR were less cytotoxic that sodium hypochlorite (NaOCl) and showed a lower% of DNA in the tail, in comparison with H2O2 (positive control − C+). In the post-irrigation microbiological sample, all the irrigating protocols were more effective than C+ (without irrigation). NaOCl/saline, NaOCl/saline/AgNPs-PVA and NaOCl/saline/FAR led to complete bacterial elimination (p >0.05). In comparison with the initial sample, both the post-irrigation and the final samples showed microbial reduction (p < 0.05). ConclusionsAgNPs-PVA and FAR showed low cytotoxicity and genotoxicity, and exhibit potential for use as a final endodontic irrigation protocols.
Highlights
The quality of life of individuals is a topic of concern of specialists from several fields; there is no consensus in the literature about what this concept means, which may be a result of its complexity and multidimensionality
It was observed that item 11 of the WHOQoLbref presented a high modification index (LM = 718.31), indicating a high correlation with the aspect "body shape concern (BSQ-8B)”, and in this way, we opted for exclusion of this item
Women who are less concerned with body shape, who do less emotional eating, perceive themselves competent in their behaviors to manage their own health, study during the day, have better expectations, perform well, who do not think about giving up on the course they attend, and who do not consume medications due the pressure of their studies have a better quality of life
Summary
The quality of life of individuals is a topic of concern of specialists from several fields; there is no consensus in the literature about what this concept means, which may be a result of its complexity and multidimensionality. Some research has used the term based on the meaning of it proposed by the World Health Organization (WHO) that sees it as the individual’s perceptions about his or her goals, expectations, patterns and concerns, and his or her position in life in the context of culture and systems of values in which they live [1]. The quality of life group of the WHO (WHOQOL) developed the World Health Organization Quality of Life Questionnaire (WHOQoL) with the collaboration of researchers from several countries. This instrument was created to evaluate the main domains related to the quality of life of people in different cultures. To aid in broad epidemiological studies, a shorter version (WHOQoL-bref) was proposed by the WHOQOL group [1], and this has been commonly used by researchers
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