Cytotoxicity evaluation of Active GP and Resilon cones in vitro

Abstract

This study was done to evaluate the cytotoxicity of Activ GP and Resilon cones in an in vitro cell culture system. Gutta-percha (GP), Activ GP, and Resilon cones were tested in this study. L929 cells were seeded into 96-well plates at 3 x 10(4) cells/well. In one set of experiments, 2-mm segments cut from the tip of GP and Resilon cones were placed into the cell culture wells and incubated for 1, 2, and 3 days. In another set of experiments, 2 20-mm segments of GP, Activ GP, and Resilon cones were incubated in 2 mL cell culture medium for 1 week. Then 100 microL elutes were tested for 24 and 48 h. Cell viability was evaluated by MTT assay. Data were analyzed using 1-way analysis of variance. When GP, Activ GP, and Resilon segments were placed into cell cultures, cell viability in the Resilon group was significantly greater than in the GP and Activ GP groups at any test time. There was no cell viability difference between the Activ GP and GP groups. When the elutes of GP, Activ GP, and Resilon was placed into cell cultures, the results were the same as using segments of the tested material. The cytotoxicity of GP and Activ GP is greater than that of the Resilon cone. There was no cell viability difference between Activ GP and regular GP. Resilon has better biocompatibility than regular GP and Activ GP cones. The cytotoxicity of Activ GP is similar to that of regular GP.