Abstract
Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. Results. All the tested materials demonstrated a reduction in cell viability (P < 0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.
Highlights
Advances in dentistry are associated with advances in dental materials and novel therapies that promote tissue regeneration without affecting the cell signaling processes mediated by undifferentiated mesenchymal cells.[1]
Physical, and mechanical properties, new versions of calcium silicatebased materials have been developed for use as capping materials
Cell culture The cells used in the present study were NIH/3T3 mouse fibroblasts (ATCC®-American Type Culture CollectionTCC, Old Town, Maryland, USA) cultured in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen®, Carlsbad, California, USA)
Summary
Advances in dentistry are associated with advances in dental materials and novel therapies that promote tissue regeneration without affecting the cell signaling processes mediated by undifferentiated mesenchymal cells.[1]. MTA cement has hydrophilic properties, good radiopacity, low solubility, high pH, antimicrobial activity, and the ability to expand after insertion in the place of interest.[7,8,9] the material is difficult to handle for insertion and condensation in the cavity and has a long setting time.[10,11] Due to good biological, physical, and mechanical properties, new versions of calcium silicatebased materials have been developed for use as capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24hour and 7-day analyses, respectively. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis
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