Abstract
As emerging pollutants, antibiotics are ubiquitous in the environment and pose a threat to human health, giving rise to an urgent need to assess their biological toxicity. In the present study, a cell electrochemical method based on the bromocresol violet/carbon nanotubes/glassy carbon electrode (BCP/MWCNTs/GCE) was established to evaluate the cytotoxicities of sulfamethoxazole (SMZ), ciprofloxacin (CIP), and tetracycline (TC). BCP/MWCNTs/GCE has advantages due to its excellent electrocatalytic activity for the oxidation of electroactive species of the Ctenopharyngodon idellus kidney (CIK) cells. The half-maximal inhibitory concentration (IC50) values of SMZ, CIP, and TC obtained by the electrochemical method were 831.51μM, 354.98μM, and 184.51μM, which were lower than those of the traditional methyl-thiazolyl-tetrazolium (MTT) assay (907.47μM, 414.87μM, and 208.11μM). These results indicate the higher sensitivity of the electrochemical method. This study provided a sensitive tool for the cytotoxicity evaluation of antibiotics in the environmental toxicology field.
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