Abstract
In this study synephrine extracted from C. sinesins peels. Detected by TLC, HPLC and biosynthesized gold nanoparticles from it, then characterization of synthesize of gold nanoparticles (AuNps) by UV-visible spectroscopy Fourier Transform Infrared (FTIR), which confirm presence of AuNps in diameter size range to (9-31) nm and determined their toxicity assay on lymphoid human cell in 24, 48 and 72 hours. The finding obtained from applying gold nanoparticles at different concentrations for 24, 48, and 72 hours showed that’s the least cytotoxicity value was in 2.351 in 10 mg/ml for Citrus sinensis extract in 24 hours. which gave indicate that synephrine extracted have not toxic effect inhibition rate of this maximum 5.511 in AuNp biosynthesized in 20 mg/ml concentration in 72 hours.
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