Cytotoxicity and antioxidant activity of combination noni (Morinda citrifolia) and periwinkle (Catharanthus roseus) extracts as anticancer candidate

Abstract

The research of herbal compounds and conventional anti-cancer compounds has been widely explored. Noni fruits are well known as an herbal medicine for cancer. In the meantime, periwinkle develops two strong alkaloids, vinblasts and vincristins. In this study, we evaluate the cytotoxicity and antioxidant activity of a combination of Noni fruits (Morinda citrifolia) and Periwinkle Madagaskar leaves (Catharanthus roseus), which in this study called Moriseus extract as an anticancer drug candidate. Noni fruit ethanol extracts and periwinkle leaf. DPPH tests were used to assess the antioxidant of the extracts. The cytotoxicity of the MCF-7 breast cancer extracts was performed using the MTT procedure. In a nutshell, cells were seeded in 96 well plates at various extract concentrations. Cells were incubated for 24 hours and tetrazolium salt solution measured their viability. After 2 h medium aspiration, DMSO was applied and the absorbance with an ELISA plate reader was estimated at 540 nm. Cytotoxicity was considered when a decrease in cell survival of more than 50 percent was observed. Combination Noni (M. citrifolia) and Periwinkle (C. roseus) extract significantly decreased the viability of MCF7 cells in a concentration-dependent manner and have IC value 53.84 ppm with the scavenging activity of combination extract are 69.91%. Combination M. citrifolia and C. roseus extract have strong cytotoxicity and antioxidant activity which could be high potential as a candidate of anticancer drug.