Cytotoxicity and action mechanisms of polycyclic aromatic hydrocarbons by a miniature electrochemical detection system.

Abstract

The effects of six polycyclic aromatic hydrocarbons (PAHs) on the activity of V79 cells were studied by using a miniature electrochemical system based on graphene oxide quantum dots and multiwall carbon nanotubes modified anodized screen printed carbon electrode. The cytotoxicity sequence of PAHs on V79 cells was different with guanine/xanthine (G/X), adenine (A), hypoxanthine (HX), and the end product of purine nucleotide catabolism, uric acid (UA), as biomarkers. The IC50 values measured with UA as the biomarker were the lowest, indicating that UA in cells was more sensitive to PAHs. The cytotoxicity sequence with G/X as the biomarker was the same as that of the MTT assay: pyrene > phenanthrene > benzo[a]pyrene > fluoranthene > fluorene > naphthalene. The cytotoxicity sequences measured by different biomarkers varied, which related to different structures that may influence the expression of the cellular aryl hydrocarbon receptor, gap junctional intercellular communication, and p53 protein. PAHs with different structures played varied roles in cell development and differentiation. Additionally, the electrochemical method was more sensitive than the MTT assay. The miniature electrochemical system enabled the simultaneous detection of four signals in cells, providing more information for multi-parameter evaluation and toxic mechanism study of PAHs and other pollutants.