Cytotoxicities of a linoleic acid hydroperoxide and its related aliphatic aldehydes toward cultured human umbilical vein endothelial cells

Abstract

An assay method for the quantification of the cytotoxicities of various agents toward cultured human endothelial cells was developed using Earle's solution as an incubation medium. By this method, the cytotoxicities of a linoleic acid hydroperoxide (LOOH) and its related aliphatic aldehydes toward human umbilical vein endothelial cells were investigated. Saturated aldehydes, pentanal, hexanal and 9-oxononanoic acid, are nontoxic; α,β-unsaturated aldehydes, 2-hexenal, 2-heptenal, 2-octenal and 2-nonenal, are toxic only at high concentrations; LOOH and α,β-unsaturated aldehydes with a hydroxy group or an additional double bond, 4-hydroxy-2-nonenal, 2,4-nonadienal and 2,4-decadienal, are highly toxic. In particular, 2,4-decadienal, whose 50% lethal concentration is 9 μM, is the most injurious. The cytotoxicities of LOOH and its related aldehydes were found to be much reduced in growth medium containing serum, growth factors, heparin, amino acids and vitamins.