Cytotoxic T cell responses to a syngeneic tumour: conditions for primary activation in vitro and biological activity in vivo.

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In vitro T cell activation against alloantigens requires antigen presentation to the responsive cell in combination with an inductive signal, termed the lymphocyte costimulator. This inductive signal can be provided by allogeneic stimulator cells or can be provided exogenously using the supernatant from Con A-activated spleen cells (CS) as a source of the costimulator (1,2). Unlike responses to alloantigens, cocultivation of the P815 tumour of DBA/2 mice with syngeneic lymph node cells results in the generation of little cytotoxic activity which is detected only at high effector to target cell ratios and after long incubation times (3,4). We have recently reported that the addition of CS to in vitro cultures allows significant cytotoxic T cell activation of DBA/2 lymphocytes to the syngeneic tumour P815 (5). In this report the requirement for CS in the generation of P815 reactive cells is further characterized and preliminary studies on the biological effectiveness of these activated cells in controlling the growth of P815 in DBA/2 mice are presented.KeywordsSpleen CellCytotoxic ResponseResponsive CellSyngeneic TumourAustralia IntroductionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.