Cytotoxic Steroids from The Stem Bark of Chisocheton cumingianus (Meliaceae)

Dewa Gede Katja,Unang Supratman,Rani Maharani,Nurlelasari Nurlelasari,Tri Mayanti,Euis Julaeha,Khalijah Awang,Kindi Farabi,Desi Harneti,Ace Tatang Hidayat

doi:10.20884/1.jm.2017.12.1.257

Dewa Gede Katja, Unang Supratman + Show 8 more

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https://doi.org/10.20884/1.jm.2017.12.1.257

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Abstract

Three cytotoxic steroids, stigmasterol (1), stigmast-5-en-3b-ol (2) and b-sitosterol-3-O-acetate (3) were isolated from the stem bark of Chisocheton cumingianus. The chemical structures of those compounds were identified based on spectroscopic data and by comparison with those data previously reported. All of the compounds isolated were evaluated for their cytotoxic effects against P-388 murine leukemia cells in vitro. Compounds 1-3 showed cytotoxicity activity against P-388 murine leukemia cells with IC50values of 12.4, 60.8, and ÃÂ 100 mg/mL, respectively.

Highlights

The Chisocheton genus belongs to the Meliaceae family is a second largest genus in the family of Meliaceae comprising more than 50 plant species and distributed in Nepal, India, Burma, Myanmar, South China, Thailand, Malaysia, Papua New Guinea and Indonesia (Vossen and Umali, 2002)
Chisocheton species, we found that n-hexane and ethyl acetate extracts of the stem bark of C. cumingianus exhibited a moderate cytotoxic activity against P-388 murine leukemia cells with IC50 value of 16.9 and 19.9 g/mL, respectively
We report the isolation and structural identification of the steroids 1-3, together with the cytotoxic activity against P-388 murine leukemia cells

Summary

INTRODUCTION

The Chisocheton genus belongs to the Meliaceae family is a second largest genus in the family of Meliaceae comprising more than 50 plant species and distributed in Nepal, India, Burma, Myanmar, South China, Thailand, Malaysia, Papua New Guinea and Indonesia (Vossen and Umali, 2002). Previous phytochemical studies on Chisocheton plants reported the presence of compounds with interesting biological activities such sesquiterpenoids As a part of our studies on anticancer candidate compounds from Indonesia Chisocheton plants, we already isolated a 7hydroxy coumarin from the stem bark of C. celibicus (Katja et al, 2015), and a 30-nor trijugin-type limonoid, chisotrijugin and lanostan-type triterpenoid, 3β-hydroxy-25ethyl-lanost-9(11),24(24′)-diene from the stem bark of C. cumingianus (Katja et al, 2016a, Katja et al, 2016b). Chisocheton species, we found that n-hexane and ethyl acetate extracts of the stem bark of C. cumingianus exhibited a moderate cytotoxic activity against P-388 murine leukemia cells with IC50 value of 16.9 and 19.9 g/mL, respectively. We report the isolation and structural identification of the steroids 1-3, together with the cytotoxic activity against P-388 murine leukemia cells

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSION