Cytotoxic potency of bioactive metabolites from an endophytic fungi Pestaloptiopsis breviseta against MCF-7 and A 549 cancer cell lines

Abstract

Fungi are organisms which lack chlorophyll and play a major role in plants. Some cause the disease to the plant, some live inside it as parasites and some as endophytes. Endophytic fungi play a very important role such that some endophytes are host specific and others just reside in it. In this study, one of the frequently recurring endophytic fungi isolated were identified as Pesatloptiopsis breviseta. The maximum absorbance was recorded at 302 nm in the UV spectra. The peaks above 3000 - 3400 cm−1 in the FT-IR analysis, confirms the existence of free OH, NH, and NO2 groups. The presence of peaks at 1900 - 2900 cm−1 confirms the presence of alkyl groups attached to an aromatic moiety and confirms the presence of a double bond in the compound. The peak at 1965 cm −1 confirms the presence of unsaturation in the molecule. The NMR spectra confirm the presence of an aromatic compound. GC-MS analysis showed the maximum m/z valve at 15.5 retention time followed by 23.38 and 18.92 correspond to the compounds Neoisolongifolane, hydroxy-, 1, 2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester and 8-Octadecenoic acid, methyl ester respectively. From the MTT assay, the percentage of cell viability based on IC50 value was observed. From the assay, it was proved that on using different concentrations, the viability of cells was minimum at 100μl. Thus this study shows that the endophytic fungi can be served as a source for the production of bioactive metabolites, with which the pharmaceutical products can be developed.