Abstract
A new lignan from the non-transformed root in vitro cultures of Phyllanthus amarus was isolated. The structure of the compound was established on the basis of one- and two-dimensional NMR, as well as mass spectrometry data, as 7'-oxocubebin dimethylether (1,4-bis(benzo[d][1,3]dioxol-5-yl)-2,3-bis(methoxymethyl)butan-1-on). The non-transformed root cultures of P. amarus showed to be a selective source of this compound. The lignan revealed strong cytotoxic activity against HeLa cell line with an IC50 value of 3.8 µg/mL.
Highlights
IntroductionPhyllanthus amarus Schum. and Thonn. (Euphorbiaceae) is widely distributed in tropical and subtropical regions of the world and has a long history in traditional therapeutic systems of Asia and
Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) is widely distributed in tropical and subtropical regions of the world and has a long history in traditional therapeutic systems of Asia andSouth America as a medicinal plant [1]
The research presented describes the isolation, structure elucidation, and cytotoxic activity determination of an unknown lignan-type compound, which is selectively accumulated in non-transformed roots of P. amarus obtained in vitro
Summary
Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) is widely distributed in tropical and subtropical regions of the world and has a long history in traditional therapeutic systems of Asia and. It has been reported that mainly lignans, next to ellagitannins, are responsible for the hepatoprotective and antihepatitis B activity of this plant [4,5,6]. The results showed that the hairy root extract displayed linear concentration- and time-dependent cytotoxicity towards the MCF-7 cell line [15]. None of these studies provide detailed information regarding the chemical composition of in vitro cultivated roots of P. amarus. The research presented describes the isolation, structure elucidation, and cytotoxic activity determination of an unknown lignan-type compound, which is selectively accumulated in non-transformed roots of P. amarus obtained in vitro
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