Abstract
The objective of this study was to develop a validated high-performance thin layer chromatography (HPTLC) method for the concurrent analysis of two diterpenes Sugiol (compound 1) and 11,14-dihydroxy-8,11,13-abietatrien-7-one (compound 2) in Plectranthus barbatus chloroform extract (PBCE) and to investigate cytotoxicity of both compounds. The chromatographic estimations were carried out using toluene : ethyl acetate : formic acid (8.2:1.3:0.5 v/v/v) as mobile phase. The compact spots of biomarkers were scanned at λmax= 275 nm. Cytotoxic evaluation of both compounds was performed using follicular thyroid cancer cells (FRO cells). A well resolved, compact and intense peaks of compound 1 (Rf = 0.550 ± 0.001) and compound 2 (Rf = 0.700 ± 0.002) were recorded. The proposed method for both compounds was recorded as simple, linear, precise (% RSD = 1.02-1.25), accurate (98-100%), robust and sensitive for the analysis of both compounds. Cytotoxicity evaluation showed significant cell cytotoxicity at 100 μg/mL concentration against the FRO cancer cells after 72 h of incubation. Both compounds were recorded as cytotoxic, however compound 1 showed significant cytotoxic effects. The proposed HPTLC method was found to be suitable for routine analysis of these two biomarkers in chloroform extract of P. barbatus. Both compounds were found to be cytotoxic against FRO cancer cells. KEY WORDS: Diterpenes, Sugiol, Plectranthus barbatus, Cytotoxicity, HPTLC Bull. Chem. Soc. Ethiop. 2018, 32(3), 407-419.DOI: https://dx.doi.org/10.4314/bcse.v32i3.1
Highlights
Plectranthus barbatus Andr. belonging to family Lamiaceae, is a perennial herb native to the India and tropical Africa, which was naturalized in the Egypt, Saudi Arabia, Pakistan, Sri Lanka, Brazilian Northeast and Southeast regions [1,2,3]
Compound 1 could be formulated in different dosage forms for the chemoprevention of cancers in future studies
The maiden high-performance thin layer chromatography (HPTLC) method developed for the concurrent analysis of cytotoxic biomarkers sugiol and 11,14-dihydroxy-8,11,13-abietatrien-7-one in the P. barbatus extract may be further employed in the analysis of these biomarkers in extracts of other species of same genus as well as different genus to establish the chemotaxonomic relationship
Summary
Plectranthus barbatus Andr. belonging to family Lamiaceae, is a perennial herb native to the India and tropical Africa, which was naturalized in the Egypt, Saudi Arabia, Pakistan, Sri Lanka, Brazilian Northeast and Southeast regions [1,2,3]. Belonging to family Lamiaceae, is a perennial herb native to the India and tropical Africa, which was naturalized in the Egypt, Saudi Arabia, Pakistan, Sri Lanka, Brazilian Northeast and Southeast regions [1,2,3]. This plant has a long history of uses by traditional Hindu and Ayurvedic medicines as well as in the folk medicine of Brazil, tropical Africa and China with potential benefits [4]. The aqueous extract of P. barbatus showed significant hypotensive, antispasmodic and hypoglycemic effects [21]. The aqueous extract of stem and leaves has been reported to enhance the intestinal transit in mice, protected against gastric lesions induced
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