Abstract
L-asparagine is an enzyme widely identified as one of the most potential anticancerous drugs. This enzyme is poorly bioavailable, so its pharmaceutical uses are limited as an anticancer. L-asparaginase is an important chemotherapeutic drug used to treat a variety of malignant tumors. Lactic acid bacteria may exhibit high L-asparaginase productivity, efficiency, and anticancer activity against breast cancer cell lines. The L-asparaginase was extracted and purified in multiple processes, including precipitation with (NH4)2SO4 (70%), DEAE-cellulose ion exchanger chromatography, and Sephadex G 150 filtration. The specific activity was 40 U/mg, and the purification fold was 25.6, yielding 51%. The cytotoxic effect of the enzyme on treated cells was dose-dependent, with an IC50 value of 4.305 IU/ml. Keywords: Cytotoxic effects, L-asparagine, Lactobacillus reuteri, breast cancer cell line
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