Cytotoxic Effects of Glycyrrhiza glabra L., Morus nigra L. and Urtica urens L. Extract against the Human Hepatocarcinoma HepG2 and Mouse L20B Cell Lines

Ban Abdul Jabbar Sidkey,Ayyad W Al-Shahwany,Raghad Abdullah Hassan

doi:10.24126/jobrc.2017.11.1.493

Abstract

The present study aimed to study the cytotoxic effects of three Iraqi traditional medical plants extracts using tissue culture teqnique on the human hepatocarcinoma HepG2 and mouse cell L20B cell lines. Glycyrrhiza glabra L. root, Morus nigra L. and Urtica urens L. leaves were extracted using 99% ethanol solvent. Seven crude concentrations were prepared by serial dilution, with concentrations of 3.9, 7.81, 15.62, 31.25, 62.5, 125 and 250 mg/ml, respectively. These were added to the microtiter plate containing 1x105 cells/well and 200 µl of the medium. The seven concentrations were used in triplicate to investigate their cytotoxic and anti-proliferative effects. The analysis of chemical composition of these plant extracts were determined by fast liquid chromatography (FLC). The extract of Urtica urens L showed the highest potent cytotoxicity in the HepG2 and L20B cell lines, while both G. glabra L. and Morus nigra L. crude extracts showed the lowest cytotoxicity. All concentrations of crude extracts showed different cytotoxic activity in vitro.

Highlights

Cancer chemoprevention is the use of natural, artificial or biologic compounds to reverse, suppress or avoid the development of invasive cancer
Phytochemicals are attractive increasingly significant sources of chemo preventive agents, as they can reveal their useful potential at all stages of tumor configuration [1,2]
Studies have shown that the extract of it has a growth inhibitory effect on breast cancer [8]

Summary

Introduction

Cancer chemoprevention is the use of natural, artificial or biologic compounds to reverse, suppress or avoid the development of invasive cancer. Phytochemicals are attractive increasingly significant sources of chemo preventive agents, as they can reveal their useful potential at all stages of tumor configuration [1,2]. The disease-prevention properties of fruits and vegetables are attributed to the biological activities of the dietary fiber, vitamins, minerals and phytochemicals in the plants, many studies suggest the protective effects of fruits and vegetables against chronic diseases are due in large part to the phytochemical content of the plants [6,7]. Studies have shown that the extract of it has a growth inhibitory effect on breast cancer [8]

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