Cytotoxic effects of fresh aloe vera gel mediated silver nano composite

Abstract

Aim: To evaluate the cytotoxic effect if fresh aloevera gel mediated silver nano composites Materials And Methods: A 10 gram portion of thoroughly washed fresh cut aloe vera was used in this study. 10 gram of the pulp of the leaf was extracted and was used for the experiments. 10 gram of silver nitrate powder was dissolved in 40 ml of distilled water. To this 40 ml of distilled water the fresh aloe vera extract was added. The solution was placed in the shaker and an observation was made for every two hours for analysing the synthesis of nanoparticles under UV visual spectroscopy . This was followed by the placement of the solution onto the magnetic stirrer for the formation of the nanoparticle .The mixture is then centrifuged at 8000 rpm for 10 minutes and then the nano particles were collected .The resulting solution was further characterised by the TEM measurements. The cytotoxicity was tested by seeding brine shrimps into a 6 well plate. Various concentration of silver nitrate solution was added on the mixture.