Cytotoxic Effects of Bisphenol A as an Endocrine Disruptor on Human Lymphocytes

Abstract

Background: Endocrine compounds, such as Bisphenol A (BPA), stimulate or inhibit the activities of hormones, nuclear receptors in the central nervous system, liver and other organs. They may be disposed of in the environment inadverdently around industrial sites. The aim of this study was to evaluate the cytotoxic effects of BPA on human lymphocytes in culture at varying concentrations. Methods: 0.1 mL heparinized 0.2 mL peripheral blood taken from a healthy male and a female were plated in culture media under sterile conditions. To prepare the reference dose at a concentration of 0.05 mg/mL, 0.027g BPA was dissolved in 1 L dimethyl sulfoxide and the highest dose of 50 μg/mL BPA solution was prepared. After separating the stock solution, 50 μg/mL BPA was diluted to prepare 20, 10 or 5 μg/mL doses. Results: After 24 h of incubation, abnormal cell±Standart Error (%)[AC±SE (%)] 1.10±1.0, chromosomal aberration/cell±Standart Error (CA/cell±SE) 0.025±0.01 was determined in control group, and AC±SE (%) 2.00±0.98 in control group. After 48 h of incubation 0.98, CA/cell±SE was found to be 0.020±0.01. After 24 and 48 h of incubation, AC±SE (%) and CA/cell±SE ratios were 30.00±3.24, 34.00±3.35 and 0.325±0.03, 0.430±0.04, respectively. Conclusion: The cytotoxic effect of BPA on human lymphocytes was investigated in this study at reference concentration and lower doses. Our findings support the fact that BPA substitutes may not be sufficiently safe for widespread use as industrial chemicals.