Cytotoxic effect of the crude alcoholic extract of Juncus rigidus on cells of the human breast cancer line MCF-7 in vitro

Abstract

This study tested the toxicological activity of the crude alcoholic extract of the vegetative part of Juncus rigidus on cells of the MCF-7. The quantitative estimation of the active compounds of the alcoholic extract (alkaloids - tannins - flavonoids - phenols) was found, as the results gave (6.06 - 7.91 - 13.84 - 16.34 mg/g) respectively. The antioxidant activity of the alcoholic extract was also calculated by DPPH method. The percentages of free radical inhibition were (9.31%, 16.43%, 19.81%,20.51%,24.31%) at concentrations (10,20,30,40,50 mg/ml) respectively. Six concentrations of (6.25, 12.5, 25, 50, 100, 200 μg/ml) of alcoholic extract of the vegetative part of the plant were tested on the growth of human breast cancer cells MCF-7 line for ( 3 ) exposure periods (24,48,72) Hr .Where the extract had a toxic effect on cell growth from the lowest used concentration (6.25 μg/ml), where the inhibition percentage was recorded (2±32.8%) for the 72-hour exposure period and for a probability level (P≤ 0.05) versus (72.3%±2) at the concentration (200 µg/ml) and for the same exposure time and probability level.