Cytotoxic Effect of Silver Nanoparticles Synthesized from Sargassum wightii on Cervical Cancer Cell Line

Abstract

The biological synthesis of the metallic nanoparticle is gaining more interest, as it is more reliable and eco-friendlier. Recently, macroalgae are widely used in pharmaceutical research for their known biological activities. The green synthesized silver nanoparticles (AgNPs) were characterized using UV–visible spectrophotometer, HPLC, FTIR, SEM, and XRD methods. The surface plasmon resonance peak in absorption spectra for synthesized AgNPs showed an absorption peak at 420 nm in a UV–visible spectrophotometer. The HPLC of seaweed methanolic extract showed the presence of six secondary metabolites. The functional biomolecules such as amino acids, esters, polysaccharides, phenols, alkanes, chlorophyll, protein amide-I band, protein amide II band, carboxylic group, glycosidic linkage, carbohydrate, and protein groups responsible for the synthesis of AgNPs were characterized by FTIR. The SEM results revealed a spherical morphology for the AgNPs. The XRD pattern confirms the crystalline structure of the synthesized AgNPs. Additionally, the biologically synthesized AgNPs exhibited dose-dependent cytotoxicity against human cervical cancer cell lines and when incubated for 24 and 48 h, respectively.