Cytotoxic effect of methanolic fractions of Scrophularia oxysepala on MCF-7 human breast cancer cell line

Abstract

Event Abstract Back to Event Cytotoxic effect of methanolic fractions of Scrophularia oxysepala on MCF-7 human breast cancer cell line Mona Orangi1, Behzad Baradaran1*, Ardalan Pasdaran2, Tohid Kazemi1, Behnaz Sadat Hosseini1 and Leila Mohammadnejad1 1 Immunology Research Center, Tabriz University of Medical Sciences, Immunology, Iran 2 Department of Pharmacognosy, Faculty of Pharmacy, Guilan University of Medical Sciences, Pharmacognosy, Iran Background: Cancer is a major public health problem in both developed and developing countries. It is the second largest common disease spread world-wide. Drug toxicity and resistance to chemotherapeutic agents make a struggle to treat cancer. For this reason, initial research focuses on traditional medicines or herbal formulations. As we know, breast cancer is the most commonly occurring cancer among women (about 25%). So, the cytotoxic effect of methanolic fractions of Scrophularia oxysepala was investigated in human breast cancer MCF-7. Scrophularia oxysepala plant was collected from Eastern Azarbaijan province, Iran. This is the first time that cytotoxic effect of Scrophularia oxysepala fractions and cell death mechanism of it, is studied. Methods: The MCF-7 and L929 cells were seeded in 96-well culture plates in the presence of different concentrations (30, 50, 100, 200, 300 μg/μl) at 12, 24, 36 hours and then results were followed to determine cytotoxic effects on viability and apoptosis by MTT, TUNEL and DNA fragmentation. Result: The obtained results declared that certain concentrations have cytotoxic effect on MCF-7 cell line and induce apoptosis while L929 cells, as normal cell line, remained intact. Conclusion: Increased concentration of the fractions and treating time reduced cell viability. Our data showed that methanolic fractions of Scrophularia oxysepala have an apoptotic effect on MCF-7 cells and it might be an effective agent in cancer treatment. Keywords: Scrophularia oxysepala fractions, MCF-7, Cytotoxicity, Apoptosis, MTT assay Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Orangi M, Baradaran B, Pasdaran A, Kazemi T, Hosseini B and Mohammadnejad L (2013). Cytotoxic effect of methanolic fractions of Scrophularia oxysepala on MCF-7 human breast cancer cell line. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00591 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Behzad Baradaran, Immunology Research Center, Tabriz University of Medical Sciences, Immunology, Tabriz, Iran, behzad_im@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers Mona Orangi Behzad Baradaran Ardalan Pasdaran Tohid Kazemi Behnaz Sadat Hosseini Leila Mohammadnejad Google Mona Orangi Behzad Baradaran Ardalan Pasdaran Tohid Kazemi Behnaz Sadat Hosseini Leila Mohammadnejad Google Scholar Mona Orangi Behzad Baradaran Ardalan Pasdaran Tohid Kazemi Behnaz Sadat Hosseini Leila Mohammadnejad PubMed Mona Orangi Behzad Baradaran Ardalan Pasdaran Tohid Kazemi Behnaz Sadat Hosseini Leila Mohammadnejad Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.