Abstract
Ten compounds, including soulameanone (1), isobruceine B (2), 9-methoxy-canthin-6-one (3), bruceolline F (4), niloticine (5), octatriacontan-1-ol (6), bombiprenone (7), α-tocopherol (8), inosine (9), and apigenin 7-O-β-D-glucopyranoside (10), were isolated from the leaves, stems, and roots of Brucea mollis Wall. ex Kurz. Their structures were determined using one-and two-dimensional NMR spectroscopy and mass spectrometry. All compounds were evaluated for their cytotoxic activity against KB (human carcinoma of the mouth), LU-1 (human lung adenocarcinoma), LNCaP (human prostate adeno-carcinoma), and HL-60 (human promyelocytic leukemia) cancer cell lines. Compound 2 showed significant cytotoxic activity against KB, LU-1, LNCaP, and HL-60 cancer cells with IC50 values of 0.39, 0.40, 0.34, and 0.23 μg/mL, respectively. In addition, compounds 3 and 5 showed significant cytotoxic activity against KB, LU-1, LNCaP, and HL-60 cancer cells with IC50 values around 1–4 μg/mL. Compounds 9-methoxycanthin-6-one (3) and niloticine (5) have been discovered for the first time from the Brucea genus.
Highlights
The results of the present study demonstrated that the methanol extract and n-hexane fraction from B. mollis leaves showed strong cytotoxic activity against three cancer cell lines: LU-1, Hep G2, and MCF-7 [6]
We report the bioactivity-guided isolation of ten compounds, i.e., soulameanone (1), isobruceine B (2), 9-methoxycanthin-6-one (3), bruceolline F (4), niloticine (5), 1-octatriacontan-1-ol (6), bombiprenone (7), α-tocopherol (8), inosine (9), and apigenin 7-O-β-D-glucopyranoside (10), from the leaves, stems, and roots of B. mollis
As compared with data reported by Polonsky et al, 1980, 1 was determined to be soulameanone, a quassinoid isolated from Soulamea muelleri [7]
Summary
The results of the present study demonstrated that the methanol extract and n-hexane fraction from B. mollis leaves showed strong cytotoxic activity against three cancer cell lines: LU-1 (human lung adenocarcinoma), Hep G2 (liver hepatocellular carcinoma), and MCF-7 (human breast adenocarcinoma) [6]. We report the bioactivity-guided isolation of ten compounds, i.e., soulameanone (1), isobruceine B (2), 9-methoxycanthin-6-one (3), bruceolline F (4), niloticine (5), 1-octatriacontan-1-ol (6), bombiprenone (7), α-tocopherol (8), inosine (9), and apigenin 7-O-β-D-glucopyranoside (10), from the leaves, stems, and roots of B. mollis. The 13C and DEPT-NMR (90 and 135) spectra of 2 showed 22 carbon signals, including four methyls, two methylenes, nine methines, and seven non-hydrogenated carbons.
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