Abstract
RESUMOObjetivo: investigar o potencial citotóxico, antimicrobiano e cicatrizante de extratos das folhas, galhos e caule da J. gossypiifolia L. Método: estudo quantitativo, experimental. Os extratos foram obtidos por maceração em etanol, concentrados em evaporador rotatório e secos em dessecador a vácuo. Na análise, realizaram-se testes de prospecção fitoquímica; citotoxidade; microdiluição em caldo e Scratch assay. Entre os metabólitos detectados, estiveram: taninos, esteroides, flavonoides, flavonas, xantonas. Resultados: o extrato do caule apresentou viabilidade celular acima de 80%. As folhas foram moderadamente citotóxicas e os galhos apresentaram ausência de viabilidade celular. Os extratos inibiram o crescimento de S. aureus, S. epidermidis e P. aeruginosa em diferentes concentrações. O Scratch assay evidenciou que a fração metanólica das folhas propiciou a migração celular em 45% a mais do que o controle. Conclusão: estudos com esta espécie vegetal devem ser continuados para isolamento do princípio ativo, visando à produção de um fitoterápico cicatrizante de feridas. Descritores: Cicatrização; Enfermagem; Citoxidade; Plantas Medicinais.ABSTRACTObjective: to investigate the cytotoxic, antimicrobial and cicatrizant potential of extracts of leaves, branches and stem of J. gossypiifolia L. Method: quantitative, experimental study. The extracts were obtained by maceration in ethanol, concentrated in a rotary evaporator and dried in a vacuum desiccator. In the analysis, phytochemical prospecting; cytotoxicity; microdilution in broth and Scratch assay tests were performed. Among the detected metabolites, were: tannins, steroids, flavonoids, flavones, xanthones. Results: the stem extract presented cell viability above 80%. The leaves were moderately cytotoxic and the branches showed no cell viability. The extracts inhibited the growth of S. aureus, S. epidermidis and P. aeruginosa at different concentrations. Scratch assay showed that the methanolic fraction of the leaves allowed the cellular migration in 45% more than the control. Conclusion: studies with this plant species should be continued for isolation of the active principle, aiming at the production of a wound healing phytotherapic. Descriptors: Healing; Nursing; Citotoxicity; Medicinal Plants.RESUMENObjetivo: investigar el potencial citotóxico, antimicrobiano y cicatrizante de los extractos de las hojas, ramas y el tallo de J. gossypiifolia L. Método: estudio cuantitativo, experimental. Los extractos fueron obtenidos por maceración en etanol, concentrados en evaporador rotatorio y seco en desecador al vacuo. En el análisis, se realizaron pruebas de prospección fitoquímica; citotoxicidad; microdilución en caldo y Scratch asay. Entre los metabolitos detectados, estuvieron: taninos, esteroides, flavonoides, flavonas, xantonas. Resultados: el extracto del tallo presentó viabilidad celular por encima del 80%. Las hojas fueron moderadamente citotóxicas y las ramas presentaron ausencia de viabilidad celular. Los extractos inhibieron el crecimiento de S. aureus, S. epidermidis y P. aeruginosa en diferentes concentraciones. El Scratch assay evidenció que la fracción metanólica de las hojas propició la migración celular en un 45% más que el control. Conclusión: estudios con esta especie vegetal deben ser continuados para aislamiento del principio activo, visando la producción de un fitoterápico cicatrizante de heridas. Descriptores: La Curación; Enfermería; Citotoxicidad; Plantas Medicinales.
Highlights
Throughout the ages, medicinal plants have been used to treat numerous diseases and to heal wounds
This study aimed to investigate the cytotoxic, antimicrobial and healing potential of leaf, branch and stem extracts of J. gossypiifolia L
The following secondary metabolites: tannins, flavonoids, steroids and coumarins, were evidenced in the species J. gossypiigolia of this study. These data corroborate with studies that carried out phytochemical prospecting with plants of the same genus, which identified the presence of alkaloids, tannins, flavonoids, steroids and saponins, these data are similar to those found in another study.[14-15]
Summary
Throughout the ages, medicinal plants have been used to treat numerous diseases and to heal wounds. Wound care is part of the Nursing care attributions, with a large number of studies published in this area, demonstrating the important role and responsibility of nurses in this process and in the search for new ways of care, attentive to the innovations of care in this area, to improve the quality of care.[3]. In this way, the use of therapies, other than conventional therapies, such as the use of herbal medicines, extracts or, even medicinal plants in natura, for wound healing, has been increased, in recent years, by the search for active principles isolated from plants, that have an effective role in the healing process.[4]
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