Abstract
Antisera against subcellular rat lymphocyte fractions, an unfractionated lymphocyte homogenate, a purified microsomal fraction and a soluble cytoplasmic fraction were produced in rabbits. The highly cytotoxic antisera against the microsomal fraction exhibited only a low stimulatory activity. The antisera against the cytoplasmic fraction were less cytotoxic, but stimulated the DNA-synthesis in rat lymphocyte cultures very effectively. Antisera against the ULH showed high cytotoxic titers and strong stimulatory properties. The cytotoxic activity could be removed by absorption with the ULH and the microsomal fraction; the stimulatory activity was reduced only by absorption with the ULH. Absorption with the cytoplasmic fraction was ineffective. In immunofluorescence studies and with the immunoferritin technique it could be demonstrated that the microsomal as well as the soluble cytoplasmic antigen is a constituent of the lymphocyte surface membrane which is distributed in patches on the membrane in different patterns. The immunofluorescence studies indicate that thymus cells lack some antigenic determinants which are present on membranes of lymph node and spleen cells.
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Schedule a callMore From: Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
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